Figure 3.

trFRET data obtained under equilibrium conditions for the mutant AK-79(Sal)-86(Trp) in 0.3 (A), and 1.8 M (B) GndHCl and the best-fit results of global analysis employing the Haas-Steinberg model (33). The experimental donor fluorescence decay (black) in the absence (DO) and presence (DA) of an acceptor and the best-fit results (down to 1% amplitude relative to the maximum count) in the absence of acceptor (red), as well as in the presence of the acceptor (Sal) (blue); the instrument response function is also shown (green). Corresponding fitting residuals (Res.) are given (bottom of each panel) as well as their autocorrelation (Aut.; see inset). To see this figure in color, go online.