Figure 2. Motility and fruiting body formation defects of Δmcp and ΔcheA mutants.

(A) Motility was measured after 48 h. Colony spreading of each mutant was normalized with that of a ΔpilA strain [72] completely incapable of S motility, to exclude cell growth effects. Error bars indicate standard deviations. One star corresponds to p<0.05; two stars correspond to p<0.005. (B) ΔcheA fruiting body formation images at 48 h and 72 h are shown. We identified classes of mutants developing earlier or later than wild type. Pictures of Δmcp fruiting bodies are shown in Figure S2. The blue color indicates ΔcheA mutants, green Δmcp.