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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Free Radic Biol Med. 2013 Dec 4;67:426–436. doi: 10.1016/j.freeradbiomed.2013.11.026

Figure 3. HI at E22 and BH4 dependency on neuronal survival and function.

Figure 3

A. Healthy neurons % seeded: In the HI group for E22, ratio of live neurons (Cholera toxin and Rhodamine positive cells after 48h culture) divided by total cells (from flow cytometer count of initial cell suspension) show the lowest number in thalamus in a BH4 deficient environment (ANOVA p=0.0172; *p =0.0009 vs basal ganglia and p=0.0012 vs cortex, paired t-test). B. Healthy neurons % seeded With BH4 supplementation, this ratio was significantly increased in thalamus, but not in basal ganglia and cortex groups (# p=0.0004, paired t-test). C. Attachment efficiency of cells: In the HI group, the efficiency of attachment given by the percentage of attached cells (at 48 h) over the total cells obtained in supernatants at 24 h and 48 hr and attached cells show that the lowest attachment in the thalamus group (ANOVA p=0.0003; * p=0.0004 vs. basal ganglia and p=0.0021 vs cortex, n=11, paired t-test). D. Attachment efficiency of cells: Supplementation with BH4 increase the efficiency of attachment in both basal ganglia (# p=0.0398) and thalamus (# p=0.0056, n=11, paired t-tests).