Figure 6. Most regions show improvement with BH4 in neuronal survival and function at E29.
A. Healthy neurons % seeded: In E29 brains (depicted by dashed lines), the ratio of live neurons (Cholera toxin and Rhodamine positive cells after 48h culture) divided by total cells (from flow cytometer count of initial cell suspension) was not different among cortex, basal ganglia and thalamus in a BH4 deficient environment. B. Healthy neurons % seeded: With BH4 supplementation, this ratio significantly increased in both basal ganglia and cortex (# p=0.0109 and p=0.0103 respectively, paired t-test) but not in thalamus group. C. Attachment efficiency of cells: The efficiency of attachment given by the percentage of attached cells (at 48 hr) over the total cells obtained in supernatants at 24 h and 48 hr and attached cells at 48 hr showed no difference attachment among the thalamus, cortex and basal ganglia. D. Attachment efficiency of cells: Supplementation with BH4 increased the efficiency of attachment in all of the groups (#BG p=0.0084, Cortex p=0.0006, thalamus p=0.0012, paired t-test).