Figure 9. HI at E29 and BH₄-dependent mitochondrial function specific to cortex.

A. Attachment efficiency of functioning mitochondria neurons: In the HI group for E29, the ratio of attached neurons with functioning mitochondria (Rhodamine + Cholera + cells at 48 h) over total neurons with functioning mitochondria (in supernatant at 24 and 48 h plus attached at 48 h) in basal ganglia was higher than cortex in a BH₄ deficient environment (ANOVA p=0.0137; * p=0.0077 basal ganglia vs. cortex, paired t-test). B. Attachment efficiency of functioning mitochondria neurons: With BH₄ supplementation, this ratio significantly increased in cortex (# p=0.0046, paired t-test) but not in basal ganglia and thalamus. C. Attachment efficiency of high mitochondria cells: Cortex had a lower ratio of attached cells with healthy mitochondria (JC-1 red>green fluorescent cells at 48 h) to total cells with healthy mitochondria (in supernatant at 24 and 48 h plus attached at 48 h) compared to basal ganglia without BH4 supplementation (* p=0.0179, paired t-test although ANOVA not significant). D. With BH₄ supplementation, there were no increases in recovery of this ratio in any of the groups.