Skip to main content
. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Free Radic Biol Med. 2013 Dec 11;67:460–470. doi: 10.1016/j.freeradbiomed.2013.12.004

Fig. 2.

Fig. 2

Optimization of reduction conditions. (A) Anti-SSG Western blot image of cell lysates from macrophages treated with different concentrations of diamide. (B) Anti-SSG Western blot images of non-reduced and reduced cell lysates from diamide treated macrophages. All cells were treated with 1 mM diamide followed by enrichment of cellular proteins using Grx reduction cocktail or using Grx only. (C) SDS-PAGE of enriched proteins. Samples were reduced by the complete reduction cocktail or with Grx3 omitted followed by resin-assisted enrichment and protein-level elution. The reduction cocktail contains 2.5 μg/mL Grx3, 0.5 mM GSH, 1 mM NADPH, and 4 U/mL of GR.