Table 1.
Selected proteins observed with increased S-glutathionylation levels in response to H2O2 treatments.
| Function | Accession | Description | Symbol | Cys-sites | Relative abundances of SSG*
|
|||
|---|---|---|---|---|---|---|---|---|
| Ctrl | 20 μM | 100 μM | 500 μM | |||||
| Oxidoreductase | P50544 | Very long-chain specific acyl-CoA dehydrogenase | ACADVL | 238§ | 1.0 | 1.3 | 0.7 | 2.5 |
| Q569X5 | G3P-dehydrogenase | GAPDH | 154# | 0.4 | 0.6 | 2.0 | 6.3 | |
| 245 | 0.9 | 1.2 | 1.0 | 2.3 | ||||
| 150# | 0.4 | 0.4 | 2.2 | 6.6 | ||||
| 22 | 0.8 | 1.0 | 1.1 | 4.5 | ||||
| Q9CR21 | Acyl carrier protein, mitochondrial | NDUFAB1 | 140 | 0.9 | 1.1 | 1.0 | 11.0 | |
| P20108 | Thioredoxin-dependent peroxide reductase, mitochondrial | PRDX3 | 230 | 1.3 | 1.1 | 0.6 | 3.3 | |
| P08228 | Superoxide dismutase [Cu-Zn] | SOD1 | 7@ | 0.8 | 1.0 | 1.3 | 1.7 | |
| P10639 | Thioredoxin | TXN | 73@ | 1.0 | 1.2 | 0.8 | 9.7 | |
| Kinases | O09110 | Dual specificity mitogen-activated protein kinase kinase 3 | MAP2K3 | 29 | 1.0 | 1.1 | 0.8 | 4.3 |
| P63085 | Mitogen-activated protein kinase 1 | MAPK1 | 159 | 1.0 | 1.0 | 1.0 | 3.5 | |
| P28867 | Protein kinase C delta type | PRKCD | 507@ | 1.0 | 1.1 | 0.9 | 2.0 | |
| 127 | 1.1 | 0.8 | 1.1 | 15.9 | ||||
| Other enzymes | P20108 | Glycine amidinotransferase, mitochondrial | GATM | 407# | 1.0 | 1.1 | 0.9 | 1.6 |
| Q6PAV2 | Probable E3 ubiquitin-protein ligase HERC4 | HERC4 | 1025# | 0.5 | 1.2 | 1.3 | 3.3 | |
| Q1HFZ0 | tRNA-methyltransferase NSUN2 | NSUN2 | 321# | 0.0 | 1.6 | 1.4 | 7.6 | |
| Q7TQI3 | Ubiquitin thioesterase | OTUB1 | 91# | 0.8 | 1.0 | 1.1 | 2.8 | |
| P61080 | Ubiquitin-conjugating enzyme | UBE2D1 | 85# | 0.8 | 1.0 | 1.2 | 1.9 | |
| Q9JKB1 | Ubiquitin carboxyl-terminal hydrolase isozyme L3 | UCHL3 | 95# | 0.0 | 0.9 | 2.1 | 18.5 | |
| Ion channels | Q8R429 | Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 | ATP2A1 | 349@ | 0.9 | 0.9 | 1.2 | 1.8 |
| Heat shock proteins | P07901 | Heat shock protein HSP 90-alpha | HSP90AA1 | 598§ | 1.0 | 0.7 | 1.2 | 2.1 |
| P11499 | Heat shock protein HSP 90-beta | HSP90AB1 | 590§ | 1.0 | 1.1 | 0.9 | 1.9 | |
| HSP90AB1 | 366 | 1.1 | 0.9 | 1.1 | 2.5 | |||
| Cytoskeletal proteins | Q6ZWM3 | Actin, cytoplasmic 1 | ACTB | 257@ | 0.6 | 1.0 | 1.5 | 5.4 |
| ACTB | 272@ | 0.8 | 1.4 | 0.7 | 4.1 | |||
| P10107 | Annexin A1 | ANXA1 | 324 | 0.9 | 1.2 | 0.9 | 2.5 | |
| Apoptosis | P29452 | Caspase-1 | CASP1 | 284# | 1.3 | 0.9 | 0.8 | 12.4 |
#
Known active-sites of the enzymes.
§
Previously reported sites of S-nitrosylation.
*
Cells were treated with different concentrations of H2O2 and the quantified SSG levels for each Cys-site were normalized against the average level across the first three conditions.