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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Free Radic Biol Med. 2013 Oct 17;67:131–138. doi: 10.1016/j.freeradbiomed.2013.10.015

Figure 3.

Figure 3

The nuclear ratio of phosphorylated to total eEF2 is higher in diphthamide-deficient cells. A and B. Subcellular fractionation and immunoblot analysis for total and phosphorylated eEF2 were performed on WT cells (A) and diphthamide-deficient cells (B), treated with CH for 3 h. α-tubulin and hnRNP were used as cytoplasmic and nuclear markers, respectively. Optical density of the eEF2 bands phosphorylated/total in cytoplasm and nucleus were normalized to a control (α-tubulin or hnRNP). Values are the mean and SEM of 4 experiments. *p<0.05 and ** p<0.01 versus the cytoplasmic control value, †††p<0.001 versus the nuclear control value. ‡‡‡ p<0.001 versus the cytoplasmic control value.