Table 3. Expression of AvrLm1, AvrLm6, AvrLm4-7 and LmCys2 in axenic culture of mycelium following ectopic integration in GC-isochores of the genome.
| Isolate/Transformanta | Mean normalised expression during in vitro growthb | Ratio of expression transformant/WT |
| LmCys2 expression | ||
| v23.1.3 | 2.84E-04 | |
| v29.3.1-LmCys2-4 | 3.96E-03 | 13.94 |
| v29.3.1-LmCys2-15 | 1.19E-02 | 41.87 |
| AvrLm4-7 expression | ||
| V23.1.3 | 2.36E-04 | |
| NzT4-AvrLm4-7-3 | 4.85E-02 | 205.69 |
| NzT4-AvrLm4-7-7 | 1.86E-02 | 78.81 |
| NzT4-AvrLm4-7-9 | 7.23E-03 | 30.7 |
| NzT4-AvrLm4-7-16 | 2.43E-02 | 103.05 |
| NzT4-AvrLm4-7-18 | 1.66E-02 | 70.55 |
| NzT4-AvrLm4-7-25 | 6.61E-02 | 280.66 |
| NzT4-AvrLm4-7-27 | 7.49E-03 | 31.81 |
| NzT4-AvrLm4-7-28 | 1.01E-01 | 429.97 |
| AvrLm6 expression | ||
| v23.1.3 | 1.15E-04 | |
| v29.3.1-AvrLm6-4 | 1.47E-01 | 1269.81 |
| v29.3.1-AvrLm6-5 | 3.55E-03 | 30.75 |
| v29.3.1-AvrLm6-11 | 1.70E-03 | 14.71 |
| AvrLm1 expression | ||
| v23.1.3 | 1.44E-03 | |
| v29.3.1-AvrLm1-8 | 1.37E+00 | 955.83 |
| v29.3.1-AvrLm1-4 | 1.12E-02 | 7.79 |
| v29.3.1-AvrLm1-12 | 4.88E-02 | 33.93 |
a
AvrLm1, AvrLm6, AvrLm4-7 and LmCys2 are in AT-isochores in the wild type (v23.1.3) isolate but inserted in GC-isochores in transformants (v29.3.1-AvrLm1-x, v29.3.1-AvrLm6-x, NzT4-AvrLm4-7-x or v29.3.1-LmCys2-x). Their expression was measured by qRT-PCR.
b
Gene expression levels are relative to β-Lmtubulin and calculated as described [94]. Each value is the average of two biological replicates (two extractions from different biological material) and two technical replicates (two RT-PCRs).