Table 1. Summary of anti-RBPJ ChIP-seq results.
Number of Peaks | RBPJ motif | CTCF motif | RBPJ or CTCF | RBPJ and CTCF | |
Asynchronous Specific1 | 1851 | 381 (21%) | 255 (14%) | 596 (32%) | 40 (2%) |
Mitotic Specific1 | 545 | 26 (5%) | 9 (2%) | 33 (6%) | 2 (0.4%) |
Common2 | 2736 | 382 (14%) | 490 (18%) | 800 (29%) | 72 (3%) |
A specific peak was defined as having enrichment within a 200 bp region of more than 4-fold between the two cell populations and a Poisson enrichment p-value<0.0001.
A common peak was defined as having similar enrichment between mitotic and asynchronous peaks. As we observed peak shifting (see Figure 4), common peaks were centered on asynchronous peaks. If centered on mitotic peaks, 8.2% and 8.4% of common peaks contain RBPJ and CTCF motifs, respectively.