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. 2013 Nov 5;96(9):774–781. doi: 10.1097/TP.0b013e3182a1931f

FIGURE 2.

FIGURE 2

DZNep hampers the in vivo survival of pathogenic CTL. TCD-BM (6.0×106) and CD44lowCD8+ T cells (1.0×106) derived from C3H mice (H2-2b, Thy1.2) were transplanted intravenously into lethally irradiated B6 recipients (H2-2b, Thy1.1). Spleen and lymph node were collected on days 8 and 14 after allo-HSCT. Donor-derived CD8+ T cells were analyzed and calculated based on cell marker Thy1.2. A, mean±SD representative FACS analysis and absolute number of donor-derived CD8+ T cells in spleen and lymph node on day 8 (n=4 for each group) and day 14 (n=4 for each group) after transplantation. Donor-divided and nondivided CD8+ T cells were analyzed based on CFSE and cell marker Thy1.2. B, mean±SD percentage of nondivided donor-derived CD8+ T cells day 8 (n=4 for each group) in spleen after transplantation. Flow cytometry was preformed after intracellular staining of IL-2. C, mean±SD representative FACS analysis, frequency, and absolute cell number of alloreactive IL-2+ CD8+ T cells on days 8 and 14 (n=4 for each group) in spleen after transplantation. PBS (TCD-BM+CD8+ T+PBS; n=4; □); DZNep (TCD-BM+CD8+ T+DZNep; n=4; ▪). *P<0.05.