TABLE 2.

Affinity of SabA for Lewis antigens determined by surface plasmon resonance

	KD determineda,b
	Sialyl-LewisX	LewisX
SabA wild-type	19.9 μm ± 2.7	50.4 μm ± 8.2
SabA Y148A	21.1 μm ± 5.6	151.7 μm ± 30.5
SabA K152A	24.5 μm ± 2.1	62.8 μm ± 13.6
SabA Q159A	NCDIc	NCDIc
SabA Q162A	31.7 μm ± 7.1	NCDIc

^a No interaction was observed between SabA and SabA mutants with Lewis^A, Lewis^B, and Lewis^Y.

^b Data shown are mean K_D values determined from four independent experiments.

^c NCDI indicates no concentration-dependent interaction was detected (K_D > 1 mm).