FIGURE 4.

Transition rates of the Φ29 DNAP translocation step extracted from ionic current traces measured in absence of dNTP. Shown are plots of log(r₁) versus voltage (A) and log(r₂) versus voltage (B) for binary complexes formed between the wild type (blue squares), Y226F (red squares), Y390F (yellow squares), D12A/D66A (blue triangles), Y226F/D12A/D66A (red triangles), or Y390F/D12A/D66A (yellow triangles) Φ29 DNAP and DNA1. Each plotted point shows the mean ± S.E. In the absence of dNTP, the fluctuation rates between the pre-translocation and post-translocation states are fully described by a two-state model with two transition rates (8). Each plotted data point shows the means ± S.E., determined from 15–30 ionic current time traces for individual captured complexes; each time trace had a duration of 5–10 s.