TABLE 1.

Translocation rates for wild type Φ129 DNAP and mutants at 180 mV

All values are reported for data collected at 180 mV and are given with the S.E.

Enzymea	r1b	r2c
	s−1	s−1
Wild type	237.57 ± 3.88	1945 ± 21.5
Y226F	159.95 ± 2.06	1253.9 ± 9.7
Y390F	221.43 ± 2.33	1334.4 ± 9.0
D12A/D66A	214.36 ± 8.56	1830.7 ± 32.2
D12A/D66A (in Mn2+)	40.05 ± 0.88	1741.9 ± 23.8
Y226F/D12A/D66A	140.71 ± 1.81	1334.6 ± 77.3
Y390F/D12A/D66A	223.08 ± 4.6	1686.6 ± 58.8
Y390F/D12A/D66A (in Mn2+)	40.43 ± 1.74	1252.6 ± 27.6

^a All enzymes were examined in Mg²⁺ unless otherwise specified.

^b The forward translocation rate.

^c The reverse translocation rate.