TABLE 3.
Average dwell times in the pre-translocation and post-translocation states
All values are reported with the S.E.
| Φ29 DNAPa | [PPi] | Pre-translocation state mean dwell time | Post-translocation state mean dwell time |
|---|---|---|---|
| mm | ms | ms | |
| Wild type | 0 | 3.08 ± 0.07 | 0.79 ± 0.01 |
| 0.5 | 2.95 ± 0.05 | 0.82 ± 0.01 | |
| 1.0 | 3.13 ± 0.16 | 0.78 ± 0.01 | |
| 1.5 | 2.73 ± 0.04 | 0.79 ± 0.01 | |
| 2.0 | 3.13 ± 0.09 | 0.8 ± 0.01 | |
| Y226F | 0 | 4.7 ± 0.07 | 0.84 ± 0.02 |
| 0.5 | 6.13 ± 0.10 | 0.82 ± 0.01 | |
| 1.0 | 8.2 ± 0.11 | 0.85 ± 0.01 | |
| 1.5 | 10.04 ± 0.16 | 0.83 ± 0.01 | |
| 2.0 | 11.8 ± 0.12 | 0.84 ± 0.01 | |
| Y390F | 0 | 3.58 ± 0.04 | 0.97 ± 0.01 |
| 2.0 | 3.3 ± 0.04 | 0.94 ± 0.01 | |
| D12A/D66A | 0 | 3.89 ± 0.07 | 0.87 ± 0.01 |
| 2.0 | 3.65 ± 0.06 | 0.83 ± 0.01 | |
| Y226F/D12A/D66A | 0 | 5.92 ± 0.09 | 1.13 ± 0.02 |
| 2.0 | 14.96 ± 0.36 | 1.2 ± 0.03 | |
| Y390F/D12A/D66A | 0 | 3.58 ± 0.02 | 0.95 ± 0.01 |
| 2 | 3.52 ± 0.04 | 0.9 ± 0.01 |
a Complexes were formed between the indicated Φ29 DNAP enzymes and DNA1 and captured atop the nanopore at 160 mV applied potential.