FIGURE 4.

CSE down-regulates HDAC2 and increases chromatin acetylation. Shown are time courses and concentration-response relationships of effects on HDAC2 and histone acetylation in CSE-treated H292 cells. A, Western blots of nuclear HDAC2. B, deacetylation activity of HDAC measured using an ELISA-based assay. C, Western blots showing acetyl- and phospho-histone H3 (Lys-9/Ser-10) and total histone H3; acetylated (Lys-12) and total histone H4. D, Western blots showing immunoprecipitated HDAC2 alkylation with 4-hydroxy-2-nonenal (4-HNE), nitrosylation, and phosphorylation. H292 cells were treated with various concentrations (1–10%) of CSE for 6 h or for 1–6 h with 10% CSE, as indicated. Data are representative of three independent experiments, with n = 3. *, p < 0.05, ***, p < 0.001, n.s. = nonsignificant.