FIGURE 3.

PML-deficient BMDM display reduced levels of ASC in the nucleus compared with WT. 8 × 10⁶ unstimulated BMDM from PML^−/− and wild type mice were fractionated into nuclear and cytosolic compartments. Antibodies targeting lamin B and GAPDH were used as nuclear and cytosolic loading controls, respectively (left panel, middle and bottom rows). Levels of ASC in both nuclear and cytosolic fractions were analyzed by Western blot (top panel). Results are representative of three independent experiments. Densitometric analysis of ASC levels in the nucleus and cytosol of PML-deficient cells are presented relative to lamin B and GAPDH content and normalized to wild type controls (right panel).