FIGURE 5.

PML deficiency enhances levels of IL-1β in response to HSV-1 and S. typhimurium and has no effect on caspase-1-dependent pyroptosis. A, for infection with HSV-1, BMDMs were left unstimulated and mock-infected or stimulated with LPS (100 ng/ml) for 3 h and infected with HSV-1 (m.o.i. 10) for 22 h. Supernatants were analyzed by ELISA for IL-1β and TNFα. B, for S. typhimurium infection, cells were stimulated with 10 ng/ml LPS for 3 h followed by infection (m.o.i. 20) for 2 h. Supernatants were analyzed for IL-1β and TNFα by ELISA (left and middle panel) and lactate dehydrogenase cytotoxicity (right panel). Results are expressed as mean ± S.D. for triplicate determinations. N.D., not detected; N.S., not significant. Results are representative of three individual experiments, **, p < 0.01; *, p < 0.05 PML^−/− versus WT controls (CTL)