FIGURE 6.

ADAMTS-4 and ADAMTS-5 directly bind to LRP1 via different non-catalytic domains. Full-length LRP1 was coated onto microtiter plates, and binding of ADAMTS-4-1 (0–200 nm), ADAMTS-4-2 (0–1 μm), ADAMTS-4-3 (0–1 μm), ADAMTS-4-4 (0–1 μm), ADAMTS-5-2 (0–75 nm), ADAMTS-5-3 (0–0.6 μm), ADAMTS-5-4 (0–0.6 μm), or ADAMTS-5-5 (0–1 μm) in the presence of 100 μm CT-1746 was measured using an M2 anti-FLAG antibody and a horseradish peroxidase-conjugate secondary antibody as described under “Experimental Procedures.”