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Inducible conductance of transfected WT and mutant claudin-2 constructs. Constructs containing the indicated cysteine mutations in the ECL1 were expressed in MDCK II Tet-Off cells. Cells were grown in Dox+ or Dox− medium on Transwells, and transepithelial conductance was measured on day 7. Inducible conductance was calculated as GDox− minus the average GDox+ for each cell line (n = 3–6).
