TABLE 1.
Size, charge, and working concentration of the thiol-reactive reagents used in this study
Reagents are listed in order of net charge, increasing size.
Chemical name | Abbreviation | Net charge | Molecular weight | Working concentration | Stock concentration |
---|---|---|---|---|---|
g/mol | mm | ||||
2-Aminoethyl methanethiosulfonate hydrobromide | MTSEA | + | 236 | 2.5 | 100× |
[2-(Trimethylammonium)ethyl] methanethiosulfonate bromide | MTSET | + | 278 | 1 | 100× |
3-(Triethylammonium)propyl Methanethiosulfonate bromide | MTS-PTrEA | + | 334 | 1 | 100× |
2-((Biotinoyl)amino)ethyl methanethiosulfonate | MTSEA-biotin | Neutral | 382 | 1 | 5× |
2-Sulfonatoethyl methanethiosulfonate | MTSES | − | 219 | 5 | 100× |