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. 2014 Jan 16;289(10):6475–6484. doi: 10.1074/jbc.M113.536888

TABLE 1.

Size, charge, and working concentration of the thiol-reactive reagents used in this study

Reagents are listed in order of net charge, increasing size.

Chemical name Abbreviation Net charge Molecular weight Working concentration Stock concentration
g/mol mm
2-Aminoethyl methanethiosulfonate hydrobromide MTSEA + 236 2.5 100×
[2-(Trimethylammonium)ethyl] methanethiosulfonate bromide MTSET + 278 1 100×
3-(Triethylammonium)propyl Methanethiosulfonate bromide MTS-PTrEA + 334 1 100×
2-((Biotinoyl)amino)ethyl methanethiosulfonate MTSEA-biotin Neutral 382 1
2-Sulfonatoethyl methanethiosulfonate MTSES 219 5 100×