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. 2014 Jan 20;289(10):6498–6512. doi: 10.1074/jbc.M113.494542

FIGURE 4.

FIGURE 4.

Treatment with Siglec-15 antibodies increases bone mineral density in mice. Three- to 4-week-old male mice were treated with the B02 Siglec-15 antibody twice per week at 1, 3, or 10 mg/kg for 4 weeks. A, bone mineral density of long bones (left panel, right femur; middle panel, right tibia) and the lumbar vertebrae (L4–L6, right panel) were measured with a densitometer. The control group was treated similarly with a murine IgG at 10 mg/kg. B, cross-sectional images of a representative right femur (top panels) dissected from a control IgG-treated mouse (control IgG, left panel) or a mouse treated with 10 mg/kg of the B02 Siglec-15 antibody (Anti-Siglec-15, right panel). Similar cross-sectional images of the L5 vertebra from the same mice are shown in the lower panels. C, osteoclast-specific TRAP (TRAP 5b) activity was measured by ELISA in serum prepared from a terminal bleed. p values (versus the IgG treated group, n = 5/group) were calculated using Student's t test. *, p < 0.05; **, p < 0.02.