FIGURE 5.

Treatment with Siglec-15 antibody results in increased osteoclast number and osteoblast surface. A, representative photomicrographs (×10 magnification) of undecalcified mouse femur sections treated with control antibody (IgG) or anti-Siglec-15, histochemically stained with TRAP (A) and ALP (C). Scale bar, 100 μm. Histomorphometrical analysis was performed. B, N.Oc/BS, number of TRAP-positive osteoclasts per trabecular bone surface (mm). D, Ob.S/BS, ALP-positive osteoblast-surface per bone surface. p values (versus the IgG treated group, n = 5/group) were calculated using the Student's t test. *, p < 0.05.