FIGURE 10.

Wnt signaling is activated in cells derived from in Li-Fraumeni syndrome patients. LFS epithelial cells (HME50, IUSM/LFS/HME) and wild type epithelial cells (HME32) were compared. A, cell lysates were prepared, and Hsp90 ATPase activity was measured as described under “Experimental Procedures.” Mean ± S.D. (error bars) are shown, n = 6. *, p < 0.01 compared with HME32 cells. Upper panel, cell lysates were subjected to immunoblotting, and the blot was probed for Hsp90 and β-actin. B, cell lysates were subjected to immunoblotting and probed for Aha1 or β-actin. C, cell lysates were immunoprecipitated with Hsp90 antibody, and the blot was probed for Aha1 and Hsp90 as indicated. D and E, cell lysates were subjected to immunoblotting and the blots probed as indicated. F and G, cell lysates were subjected to Western blotting and probed as indicated.