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. 2014 Jan 15;289(10):6551–6564. doi: 10.1074/jbc.M113.533232

FIGURE 2.

FIGURE 2.

Antagonists of LPA1 impair osteoclast differentiation from primary culture bone marrow cell culture. A, visualization of TRAP+ multinucleated osteoclasts obtained from wild type BALB/c mice grown with serum + MCSF + RANK-L in the presence of vehicle (WT+Veh) or Ki16425 (10 μm), Denio0719 (1 μm), or VCP12249 (10 μm) (upper panels) and evaluation of number of TRAP+ multinucleated cells in % of vehicle-treated cells (lower panel). B, micrographs of bone marrow cells from wild type C57Bl/6–129/SvJ (C57Bl/6) or Lpar2−/− mice grown with serum + MCSF + RANK-L in the presence of vehicle (WT) or Ki16425 (10 μm) and stained for TRAP activity (upper panels). Number of TRAP+ multinucleated cells in % of vehicle-treated cells (lower panel). All values are the mean ± S.D. of 3 to 4 experiments. **, p < 0.01; ***, p < 0.001 versus vehicle-treated cells with the same genotype using one-way ANOVA with Bonferroni post-test. Bar scales, 100 μm.