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. 2014 Jan 30;289(10):6921–6933. doi: 10.1074/jbc.M113.524819

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — CD166 protected YAP via down-regulation of AMOT130. A and B, AMOT130 regulated YAP expression by affecting its localization. YAP expression and localization were detected by Western blotting (Bel-7402 cells) (A) and microscopic analysis (Bel-7402 and SMMC-7721 cells) (B). Arrows, cells transfected with AMOT130-HA; *, cells without successful transfection. C, YAP expression in control (infected with GFP shRNA) and Bel-7402 cells with AMOT130 knocked down (infected with sh1 and sh2, respectively) by Western blotting. D, AKT reduced AMOT130. Western blots of AMOT in control (transfected with empty plasmid) and cells as indicated with AKT overexpressed (transfected with AKT-Myc-expressing plasmid). E (a′ and b′) Western blots of AMOT130 in Bel-7402 cells with or without CD166 overexpressed (a′) or knocked down (b′). F, CD166 blocked AMOT130 interaction with YAP. In CD166-Myc-transfected Bel-7402 cells, AMOT130 was immunoprecipitated with an anti-HA antibody, and Western blotting analysis of YAP was done by an anti-FLAG antibody.