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. 2014 Jan 30;289(10):6921–6933. doi: 10.1074/jbc.M113.524819

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — CD9 interacted with CD166 to up-regulate YAP. A, Western blots of AMOT130 and YAP in Bel-7402 cells with or without CD6-FLAG or CD9-FLAG overexpressed. B, CD9 increased YAP without affecting CD166 expression. Shown are Western blots of proteins as indicated in control cells (transfected with empty plasmid) and SMMC-7721 cells with CD9 overexpressed (transfected with CD9-Myc-expressing plasmid). C, knockdown of CD9 decreased YAP expression as measured by Western blotting. D, CD9 did not affect YAP transcription as measured by qPCR with or without CD9 knocked down (control; infected with GFP-shRNA). E, CD9 bound to CD166. CD166-HA was co-transfected with CD9-Myc into Bel-7402 cells. CD166 and CD9 associations were examined by reciprocal co-IP as indicated. F, CD9 facilitated self-binding of CD166. In CD9-Myc-transfected Bel-7402 cells, CD166-HA was immunoprecipitated with an anti-HA antibody, and Western blotting analysis of CD166-FLAG was done by an anti-FLAG antibody. Error bars, S.D.