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. 2014 Jan 10;289(10):6949–6959. doi: 10.1074/jbc.M113.492132

FIGURE 2.

FIGURE 2.

Arp3 knockdown diminishes HIV-1 infection. A, transient shRNA knockdown of Arp3 inhibits HIV replication. CEM-SS cells were electroporated with indicated amounts of vector pLKO.1-Puro carrying Arp3 shRNA (shArp3) or a non-targeting shRNA (shNTC). Knockdown cells were infected with HIV-1 at 3 days post electroporation. Viral replication was measured by p24 release. B, Western blot measurement of cell lines carrying stable Arp3 knockdown. Individual shRNA Arp3 knockdown cell clones and the control shNTC cells were analyzed by Western blot using an antibody again Arp3. The same blot were also probed with an antibody again GAPDH for loading control. C, stable Arp3 knockdown diminishes HIV-1 infection. The Arp3 knockdown clones, shArp-09, shArp-10, shArp-11, shArp-12, shArp-13, and the control shNTC cells, were identically infected with HIVNL4–3 for 2 h. Cells were washed twice and then culture for 6–9 days to monitor viral replication by p24 release.