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Location of VIM in cloned embryos by immunofluorescence analysis. A, A′, and A″, immunofluorescence analysis of oocyte VIM. B, B′, and B″, location of VIM in cloned embryos at 2 h post-NT. C, C′, and C″, location of VIM in cloned embryos at 6 h post-NT. D, D′, and D″, location of injected VIM in cloned embryos at 2 h post-NT. E, E′, and E″, expression pattern of VIM in porcine fibroblasts. Green, VIM; blue, DNA. Scale bar, 50 μm.
