TABLE 2.
Ligand dissociation constants (nm)
CdG and fl-cdG dissociation constants in the absence and presence of 2 mm CaCl2 measured by microscale thermophoresis. [fl-cdG] = 60 nm, [protein] = 2 μm and 90 nm for the cdG competition experiments in the absence and presence of CaCl2, respectively. Values are rounded to 2 digits, p = 0.95 confidence intervals are given in parentheses assuming a 5% error in the individual measurements.
| fl-cdG |
cdG |
|||
|---|---|---|---|---|
| −Ca2+ | +Ca2+ | −Ca2+ | +Ca2+ | |
| YahA-EAL | 489 (281…876) | 33 (12…80) | 22 (6…107) | <1 |
| YahA-EAL(S298W) | 525 (310…883) | 48 (21…90) | 126 (15…526) | <1 |