. 2014 Jan 22;289(10):6978–6990. doi: 10.1074/jbc.M113.516195

TABLE 3.

Dimer dissociation constants in the absence and presence of cdG (in μm)

The molar cdG:protein ratio was 3:1 and divalent cation concentration 2 mm, where applicable.

Method	K_d_,00 = K₀₀⁻¹			K_d_,11 = K₁₁⁻¹
Method	−cdG, −M²⁺^a	−cdG, +Mg²⁺	−cdG, +Ca²⁺	+cdG, +Mg²⁺	+cdG, +Ca²⁺
MALS (Fig. 6C)	188 (80…1600)	36 (21…65)	13 (0.8…25)	1.1 (0.6…2.3)^b	0.6 (0.3…1.1)^b
AUC (Fig. 7)					0.4 (0.2…0.9)
Activity (Fig. 8C)				0.5 (0.47…0.54)

^a Without divalent cations.

^b Sample not fully complexed upon elution; ligand occupancy ∼0.7 as derived from combined UV/RI measurement. p = 0.95 confidence intervals are given in parentheses assuming a 5% error in the individual measurements.