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. 2014 Jan 22;289(10):6991–7002. doi: 10.1074/jbc.M113.511444

FIGURE 1.

FIGURE 1.

Effect of TSA and MGCD0103 on Npr1 gene transcription and expression. A, luciferase activity of the Npr1 proximal promoter construct −356/+55 in MMCs treated with increasing concentrations of HDACi (TSA, MGCD0103, and MC1568). B, effect of TSA (50 nm) and MGCD0103 (1 μm) on Npr1 mRNA levels in a time-dependent manner as determined by real-time RT-PCR. C, Western blot (WB) analysis of NPRA protein expression in cells treated with HDACi and β-actin expression is shown as the loading control. D, intracellular accumulation of cGMP in MMCs treated with HDACi (TSA, 50 nm; MGCD0103, 1 μm) and ANP. Bar represents the mean ± S.E. of four independent experiments in triplicates. WB, Western blot; *, p < 0.05; **, p < 0.01; ***, p < 0.001.