FIGURE 6.

Effect of HDACi on interaction of HDAC1 and -2 with Sp1 on the Npr1 promoter. A, Western blot analysis of HDAC1, HDAC2, or Sp1 complexes isolated by immunoprecipitation with anti-Sp1 antibody from HDACi-treated cells. Sequential ChIP analysis demonstrating in vivo recruitment of HDAC1 (B) and HDAC2 (C) to the Npr1 promoter by Sp1 in MGCD0103-treated and untreated cells. The intensity of DNA bands was quantified by Alpha Innotech analysis software. Data shown represent mean ± S.E. of three independent experiments. MGCD0103 (1 μm); UT, untreated; WB, Western blot; IP, immunoprecipitation.