BAMBI regulation by TLR4 signaling.
A, Bambi mRNA expression in primary mouse HSCs, Kupffer cells (KC), and hepatocytes was determined by RT-PCR. B, Bambi mRNA levels in quiescent and 5-day culture-activated HSCs, HSCs isolated from bile duct ligation (BDL) and CCl4-treated mice, and quiescent HSCs stimulated with LPS (100 ng/ml) for 4 h were determined by qPCR. C and D, BAMBI mRNA levels in LX2 cells (C) and hHSCs (D) 4 h after LPS (100 ng/ml) or TNF-α (10 ng/ml) stimulation were determined by qPCR. Un, untreated. Data represent mean ± S.D. *, p < 0.05; **, p < 0.01.