FIGURE 2.

Identification of the element in the promoter region responsible for repression of BAMBI by LPS and TNF-α. Nucleotide sequence positions are indicated relative to the transcriptional start site. LX2 cells were transfected with the indicated hBAMBI reporter constructs. 6 h after transfection, cells were treated with LPS (100 ng/ml) (A) or TNF-α (10 ng/ml) (B) for 16 h, followed by measurement for luciferase activities. The Renilla luciferase reporter was cotransfected to normalize transfection efficiency. Un, untreated. Experiments were performed three times and are shown as mean ± S.D. **, p < 0.01.