FIGURE 5.

Effect of mutation of the −3166 κB-binding site in the BAMBI promoter. A, the predicted p50 binding sequence (top panel) and designed mutant sequence (bottom panel). B, hBAMBI p-3384+82-luc, which contains an NF-κB binding site, was used to assess the functional significance of the −3166 binding site. The p-3384+82-luc construct was responsive to an LPS or TNF-α stimulus, whereas the promoter constructs harboring a mutation of the predicted −3166 κB binding site (p-3384+82-mutant-luc) abrogated the LPS- or TNF-α-induced suppression of luciferase expression. Un, untreated. Data represent mean ± S.D. **, p < 0.01.