FIGURE 3.

Reversible MORF4L1 acetylation at Lys-148 modulates its dimerization. A and B, lysine mutants and myc-tagged, wild-type MORF4L1 were coexpressed in cells. Cell lysates were subjected to myc immunoprecipitation (IP), and the precipitates were analyzed by V5 and myc immunoblot analysis. The cell lysates were analyzed with V5 immunoblotting to verify MORF4L1 mutant expression. The densitometry results of A were plotted in B. C, K148R and the acetylation mimic K148L and K148Q mutants were coexpressed with myc-tagged, wild-type MORF4L1 in the cells. The cell lysates were used for myc immunoprecipitation, followed by immunoblotting analysis as indicated. MORF4L1 mutant expression levels were analyzed by V5 immunoblotting. D, wild-type of MORF4L1 tagged with V5 or myc were expressed in cells for 40 h, and cells were treated with anacardic acid (final concentration of 50 μm) or trichastatin A (final concentration of 100 nm) for 6 h. Cell lysates were subjected to myc immunoprecipitation and followed by immunoblot analysis as indicated. Tagged MORF4L1 expression was verified by immunoblotting. These data are representative of three separate experiments.