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. 2014 Feb 3;289(10):7121–7130. doi: 10.1074/jbc.M114.548859

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Hrp38 and TBPH genetically interact to regulate flies locomotion and life span. A, a climbing ability analysis of Hrp38-silenced flies demonstrates the existence of a genetic interaction between TBPH and Hrp38. The phenotype of Hrp38 neuronal silencing becomes significantly stronger if one copy of TBPH is removed as compared with the silencing in a wild type background (Elav-GAL4, tbph^Δ23/+; Hrp38^RNAi versus Elav-GAL4, tbph+/+; Hrp38^RNAi, p < 0.01). The results of Hrp38 silencing in a TBPH sensible background further support this genetic interaction. In fact, Hrp38 silencing exacerbated the phenotype caused by TBPH silencing (Elav-GAL4, tbph^Δ23/+; TBPH^RNAi versus Elav-GAL4, tbph^Δ23/+; TBPH^RNAi/Hrp38^RNAi, p < 0.001). n = 200 flies for genotype, error bars indicate S.E. Statistics were performed using GraphPad Prism. One-way analysis of variance was performed using Bonferroni's multiple comparison test to compare the genotypes (** indicates p < 0.01; ***, p < 0.001). B, percentage of flies survivors during aging. Median lifespan is: 49 days for wild type (n = 208), 44 days for tbph^Δ23/+ (n = 126), 33 days for Elav-GAL4, tbph^Δ23/+; TBPH^RNAi (n = 150), 14 days for Elav-GAL4, tbph^Δ23/+; TBPH^RNAi/Hrp38^RNAi (n = 138), 51 days for Elav-GAL4, tbph^Δ23/+; GFP (n = 159), 32 days for Elav-GAL4, tbph^Δ23/+; Hrp38^RNAi (n = 312), and 36 days for Elav-GAL4, tbph+/+; Hrp38^RNAi (n = 235). Log rank test and p value: wild type versus tbph^Δ23/+ (p = not significant), wild type versus Elav-GAL4, tbph^Δ23/+; TBPH^RNAi (p < 0.0001), wild type versus Elav-GAL4, tbph^Δ23/+; TBPH^RNAi/Hrp38^RNAi (p < 0.0001), wild type versus Elav-GAL4, tbph^Δ23/+; GFP (p = not significant), wild type versus Elav-GAL4, tbph^Δ23/+; Hrp38^RNAi (p < 0.0001), wild type versus Elav-GAL4, tbph+/+; Hrp38^RNAi (p < 0.0001), Elav-GAL4, tbph^Δ23/+; TBPH^RNAi versus Elav-GAL4, tbph^Δ23/+; TBPH^RNAi/Hrp38^RNAi (p < 0.0001). and Elav-GAL4, tbph+/+; Hrp38^RNAi versus Elav-GAL4, tbph^Δ23/+; Hrp38^RNAi (p < 0.0001). Statistics were performed using GraphPad Prism. A log rank test was performed to compare survival distribution of the genotypes (***, indicates p < 0.001). C, RNAi treatment against Hrp38 reduced mRNA expression levels in Drosophila heads, as compared with control untreated flies.