FIGURE 1.

Distinct PMCA4 splice variant expression levels in mouse organs. A, qRT-PCR-determined relative proportions of PMCA4 splice variants in various organs of C57BL6/J mice. Bars represent mean relative proportion for each splice variant mRNA extracted from the organs of three C57BL6/J mice, normalized for cDNA conversion efficiency (EGFP) and loading (β2-microglobulin). Liver gave no PMCA4 splice variant copies upon amplification. B, immunoblots of microsomal protein extracts from various organs of C57BL6/J mice. Microsomal extracts were made from the organs of three C57BL6/J mice and resulted in nine separate immunoblots (three each for PMCA4-a/e, PMCA4b, and GAPDH); representative blots are shown. Br, brain; U, uterus; bl, bladder; M, skeletal muscle; K, kidney; S, stomach; H, heart; L, liver.