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Concentration-dependent kinetics of [14C]arginine (left) and [14C]lysine (right) uptake by yeast strain 22Δ8AA expressing the native or a mutant form of Can1. Error bars, S.E.; n = 2. The arginine and lysine transport activities of control cells (empty plasmid) were subtracted for each concentration. Curve fitting was carried out by non-linear one-site binding (competition) analysis. When not visible, error bars are smaller than the symbols. Prot, protein.
