FIGURE 8.

Conservation and protonation probability of Glu-184. A, multiple sequence alignments of 15-amino acid permeases of S. cerevisiae showing the conservation degree of Glu-184 (Can1 numbering) using ClustalW (69). These sequences were taken from the YTP database (66). B, protonation probability of Glu-184 in the OF occluded Can1 model. Two docked positions of arginine featuring two different orientations of its side chain pointing either toward Glu-184 (dashed line) or Glu-301 (solid line) were considered (see “Results”).