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. 2014 Jan 21;289(10):7232–7246. doi: 10.1074/jbc.M113.525915

TABLE 3.

Primer sequences used in this study

Primer Sequence (5′ → 3′)
5clCAN1pFL38 ACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACTCTTGTCCCTTATTAGCC
3clCAN1pFL38 TATCACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGCTAATTATACATTAGTATTAGCGTG
5-CAN1S176N AATGGTTACATGTATTGGTTTAATTGGGCAATCACTTTTGCCCT
3-CAN1S176N AGGGCAAAAGTGATTGCCCAATTAAACCAATACATGTAACCATTGGCC
NEW5CAN1-S176A AATGGTTACATGTATTGGTTTGCTTGGGCAATCACTTTTGCCCT
NEW3CAN1-S176A AGGGCAAAAGTGATTGCCCATCGAAACCAATACATGTAACCATTGGCC
5CAN1-T456S TACTGGTGGTGACAAAGTTTTCGAATGGCTATTAAATATCTCTGGTGTTGCAGGCTTTTTTGC
5CAN1-T456A TACTGGTGGTGACAAAGTTTTCGAATGGCTATTAAATATCGCTGGTGTTGCAGGCTTTTTTGC
3CAN1-T456 GATATTTAATAGCCATTCGAAAACTTTGTCACCACCAG
5CAN1-E184Q GGGCAATCACTTTTGCCCTGCAACTTAGTGTAGTTGGCCAAGTCA
3CAN1-E184Q ACTTGGCCAACTACACTAAGAACCAGGGCAAAAGTGATTGCCC
5-E184A-CAN1 GCAATCACTTTTGCCCTGGCACTTAGTGTAGTTGGCCAAG
3-E184A-CAN1 CTTGGCCAACTACACTAAGTGCCAGGGCAAAAGTGATTGC
5–433-CAN1 CATTCATCCCTGTTACATCC
3–836-CAN1 AACCTAAGAACCTCCCTTCG
5-CAN1-GFP ACCAAAGACTTTTTGGGACAAATTTTGGAATGTTGTAGCAGGAGCAGGTGCTGGTGCTGG
3-CAN1-GFP AGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCAGCTAAACAGATCTATATTACCCTG
5clLYP1pFL38 ACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTATTGCCATTGGAGAAAGCCC
3clLYP1pFL38 TATCACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGATTTGAGTACTATCGCTGGC
5-LYP1-GFP GCCTAAGAATTTATGGGAGAAATTCTGGGCTGCTGTTGCAGGAGCAGGTGCTGGTGCTGG
3-LYP1-GFP TATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGAGCTAAACAGATCTATATTACCCTG