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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Cell Microbiol. 2013 Nov 3;16(3):396–410. doi: 10.1111/cmi.12224

Figure 1. The protein product of Bab1_1492 is secreted during the infection of host cells.

Figure 1

A. Schematic representation of the screening method used to identify novel secreted virulence effectors in Brucella abortus. B. Immunofluorescence of J774 A.1 cells infected with a B. abortus strain coding for Bab1_1492-3xFLAG in a replicative vector at 4 hrs post-infection. Red, Brucella; green, Flag. C. Immunofluorescence of J774 A.1 cells infected with a B. abortus strain coding for Bab1_0740-3xFLAG in a replicative vector at 4 hrs post-infection. Red, Brucella; green, Flag. D. Western-blot with an anti-FLAG of the strains shown in panels B and C.