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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Cell Microbiol. 2013 Nov 3;16(3):396–410. doi: 10.1111/cmi.12224

Figure 5. SepA participates in the early stages of intracellular replication.

Figure 5

A. Intracellular replication of B. abortus wild type and ΔsepA deletion strains in HeLa cells determined by the gentamicin protection assay. B. Intracellular replication of B. abortus wild type and ΔsepA deletion strains in J774 A.1 cells determined by the gentamicin protection assay. C. Intracellular replication of B. abortus wild type and ΔsepA deletion strains in bone marrow derived macrophages determined by the gentamicin protection assay D. Viable bacteria of the wild type (Ba wt), Ba ΔsepA mutant and complemented strains at 4 hrs post-infection measured by an antibiotic protection assay in J774 A.1 cells with different multiplicity of infection ratios (50, 100 and 500). E. Growth curve of the wild type and the ΔsepA deletion strain in TSB. F. Complementation of the 4 hrs phenotype with the construction lacking the N-terminal periplasmic localization signal. **P<0.002 and ***P<0.001.