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. 2014 Feb 20;2014:182549. doi: 10.1155/2014/182549

Figure 7.

Figure 7

FICZ changes the expression of the Th17 associated microRNA miR-326. (a) The expression of microRNA hsa-miR-326 in CD4+ T cells cultured with anti-CD3/anti-CD28 (○) and with Th17 polarizing conditions (●) for 1, 3, and 5 days was measured by real-time PCR. Data were normalized to the expression of hsa-SNORD-44 and the microRNA expression at day 0 using the ΔΔCt-method. Data are shown as mean ± SEM; *represents significance, P < 0.05, unpaired Student's t-test after log-transformation, and n = 5–7. (b) The expression of microRNA hsa-miR-326 in CD4+ T cells cultured with anti-CD3/anti-CD28 and Th17 polarizing conditions in the presence (●) of the AHR ligands FICZ (100 nM), TCDD (5 nM), and B[a]P (100 nM) for 1, 3, and 5 days was measured by real-time PCR. CD4+ T cells treated with anti-CD3/anti-CD28, Th17 polarizing conditions, and DMSO (0.05%) were used as control (○). Data were normalized to the expression of hsa-SNORD-44 and the microRNA expression at day 0 using the ΔΔCt-method. Data are shown as mean ± SEM; *represents significance, P < 0.05, unpaired Student's t-test after log-transformation, and n = 5–7.