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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Apoptosis. 2014 Mar;19(3):451–466. doi: 10.1007/s10495-013-0942-3

Fig. 1. Inhibition of Cdk reduces IEC-6 cell proliferation.

Fig. 1

IEC-6 cells were trypsinized and equal numbers of cells were seeded in serum-containing medium. Eighteen hours later the attached cells were treated with DMSO, 1 M AZD5438, and 1 M NU6140 in serum-containing medium. Culture medium was changed everyday containing respective inhibitors and cells were incubated for 72h. (A) Phase contrast-microscopy of cells grown in the presence of DMSO, AZD5438, and NU6140 (B) Proliferating IEC-6 cells were treated with DMSO, 1 M AZD5438, and 1 M NU6140 in the presence of serum for the indicated time periods. Cell proliferation was measured by WST-1 assay as described in Methods. Values expressed are mean SE, n=3. * significantly different compared to cells grown in the presence of DMSO at indicated time points (P< 0.05), ** significantly different compared with the DMSO treated group at 24h.