(A) Caco-2 cells were treated with DMSO, 5 M AZD5438, 5 M NU6140 and 2 M CPT during proliferation for the indicated time periods. Cell lysates were analyzed for active caspase-3 using specific antibody. Actin was used as an internal loading control. (B) Caco-2 cells were treated with DMSO, 5 M AZD5438, 5 M NU6140 and 2 M CPT during proliferation for 48h. DNA fragmentation was measured by ELISA. Values expressed are mean SE, n=3. *significantly different compared to DMSO treated cells (P<0.05).