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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Apoptosis. 2014 Mar;19(3):451–466. doi: 10.1007/s10495-013-0942-3

Fig. 5. NU6140 and DFMO block CPT-induced apoptosis in proliferating IEC-6 cells.

Fig. 5

(A) IEC-6 cells were trypsinized from stock flasks and equal numbers were seeded in serum containing medium. The cells were allowed to attach overnight. Attached cells were treated with DMSO, 1 M AZD5438, 1 M NU6140, and 5mM DFMO in the presence of serum. Cells were incubated with these inhibitors for 48h in serum containing medium followed by treatment with or without 2 M CPT for 18h. Cell extracts were analyzed to determine the levels of phospho-p53 Ser15, total-p53, p21Cip1, phospho-H2AX Ser139, and active caspase-3 by western blot analysis. Actin was used as an internal loading control. (B) DNA fragmentation was measured by ELISA as described in methods. Values expressed are mean SE, n=3. *significantly different compared to cells grown in the presence of DMSO (P<0.05), **significantly different compared with cells grown in the presence of DMSO and treated with 2 M CPT (P<0.05).