Item	Item No	Recommendation
Title and abstract	1	(a) Indicate the study’s design with a commonly used term in the title or the abstract Included—title and abstract
		(b) Provide in the abstract an informative and balanced summary of what was done and what was found Included, page 2
Introduction
Background/Rationale	2	Explain the scientific background and rationale for the investigation being reported Included, page 4–5
Objectives	3	State specific objectives, including any prespecified hypotheses Included, page 5
Methods
Study design	4	Present key elements of study design early in the paper Included, page 5–7
Setting	5	Describe the setting, locations, and relevant dates, including periods of recruitment, exposure, follow-up, and data collection Included, Page 5
Participants	6	(a) Give the eligibility criteria, and the sources and methods of selection of participants. Describe methods of follow-up Included, page 5–6
		(b)For matched studies, give matching criteria and number of exposed and unexposed Not applicable
Variables	7	Clearly define all outcomes, exposures, predictors, potential confounders, and effect modifiers. Give diagnostic criteria, if applicable Included, page 5–7
Data sources/Measurement	8 *	For each variable of interest, give sources of data and details of methods of assessment (measurement). Describe comparability of assessment methods if there is more than one group Included, page 5–7
Bias	9	Describe any efforts to address potential sources of bias Not applicable, complete cohort
Study size	10	Explain how the study size was arrived at Time limited cohort
Quantitative variables	11	Explain how quantitative variables were handled in the analyses. If applicable, describe which groupings were chosen and why Described statistics section
Statistical methods	12	(a) Describe all statistical methods, including those used to control for confounding
		(b) Describe any methods used to examine subgroups and interactions
		(c) Explain how missing data were addressed
		(d) If applicable, explain how loss to follow-up was addressed
		(e) Describe any sensitivity analyses Described, statistics section
Results
Participants	13 *	(a) Report numbers of individuals at each stage of study—e.g., numbers potentially eligible, examined for eligibility, confirmed eligible, included in the study, completing follow-up, and analysed See footnote Table 1and methods section
		(b) Give reasons for non-participation at each stage
		(c) Consider use of a flow diagram
Descriptive data	14 *	(a) Give characteristics of study participants (e.g., demographic, clinical, social) and information on exposures and potential confounders
		(b) Indicate number of participants with missing data for each variable of interest
		(c) Summarise follow-up time (e.g., average and total amount) Descriptive data presented - results and Tables
Outcome data	15 *	Report numbers of outcome events or summary measures over time Outcome data presented - results and Tables
Main results	16	(a) Give unadjusted estimates and, if applicable, confounder-adjusted estimates and their precision (e.g., 95% confidence interval). Make clear which confounders were adjusted for and why they were included
		(b) Report category boundaries when continuous variables were categorized
		(c) If relevant, consider translating estimates of relative risk into absolute risk for a meaningful time period Appropriate estimates adjusted—unadjusted presented
Other analyses	17	Report other analyses done—e.g., analyses of subgroups and interactions, and sensitivity analyses
Discussion
Key results	18	Summarise key results with reference to study objectives
Limitations	19	Discuss limitations of the study, taking into account sources of potential bias or imprecision. Discuss both direction and magnitude of any potential bias
Interpretation	20	Give a cautious overall interpretation of results considering objectives, limitations, multiplicity of analyses, results from similar studies, and other relevant evidence
Generalisability	21	Discuss the generalisability (external validity) of the study results Discussion follows recommended format
Other information
Funding	22	Give the source of funding and the role of the funders for the present study and, if applicable, for the original study on which the present article is based Included, page 14

* Give information separately for exposed and unexposed groups. Note: An Explanation and Elaboration article discusses each checklist item and gives methodological background and published examples of transparent reporting. The STROBE checklist is best used in conjunction with this article (freely available on the Web sites of PLoS Medicine at http://www.plosmedicine.org/, Annals of Internal Medicine at http://www.annals.org/, and Epidemiology at http://www.epidem.com). Information on the STROBE Initiative is available at http://www.strobe-statement.org.