Figure 4.
Immunofluorescence and quantification of hippocampal vascularization. Images above of photomicrographs of CD31 stain for vascularization in the hippocampus of AD-MSC-VEGF mouse at 9 months (scale bar 100 μm). Bellow, quantification of microvessels stained by CD31 in the hippocampus at the ages of 6, 9 and 12 months in the WT-SAL, AD-SAL, AD-MSC and AD-MSC-VEGF animals (two-way ANOVA followed by Tukey’s multiple comparisons test, mean ± standard error; * P < 0.05; ** P < 0.01; *** P < 0.001, n = 5 per group).